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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: J Immunol. 2009 Aug 19;183(6):3778–3787. doi: 10.4049/jimmunol.0900843

FIGURE 10.

FIGURE 10

Ag expression during ST-OVA infection in vivo is undetectable. B6129 F1 mice were infected with 103 CFU of ST-OVA or LM-OVA i.v. Spleens were isolated under aseptic conditions and immediately frozen in a dry ice. RNA was extracted from these samples as described in Materials and Methods. Expression levels of OVA mRNA and 16S rRNA in response to ST-OVA (A) and LM-OVA (B) infection was determined and expressed as fold change to noninfected samples. Splenic bacterial burden was also plotted (C) and correlated well with 16S rRNA expression (n = 3).