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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: J Immunol. 2009 Aug 19;183(6):3778–3787. doi: 10.4049/jimmunol.0900843

FIGURE 5.

FIGURE 5

Lack of Ag presentation by ST-infected APCs is not due to virulence factors. IC-21 macrophages were seeded in 24-well-plates in RPMI 1640 plus 8% FBS. Bacteria were added at an MOI of 10 and plates were centrifuged to promote bacterial adsorption and incubated at 37°C for 15–30 min. Cells were subsequently washed vigorously and incubated with medium containing gentamicin (50 μg/ml). A, At 1 h, bacterial burden (CFU) was determined to enumerate the degree of infection. Differences in bacterial uptake were not significant (p = 0.077) as determined by one-way ANOVA. B, Subsequently, cells were washed and cultured in medium containing lower levels of gentamicin (5 μg/ml) with CFSE-labeled OT-1 splenocytes. After 4 days, cells were harvested and stained with anti-CD8α and anti-CD45.1 Abs. The degree of CD8+ T cell proliferation was evaluated by measuring CFSE dilution using flow cytometry. OVA-pep, OVA-peptide.