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. 2014 May 6;9(5):e96756. doi: 10.1371/journal.pone.0096756

Figure 3. Circulating blood volume.

Figure 3

One hour following the transfusion of biotinylated RBC, the ratio of biotinylated RBCs to native circulating RBCs was determined by flow cytometry (A, representative histogram showing 97.4% non-biotinylated RBC peak on the left and 2.6% biotinylated RBCs on the right). For 4 mice, 200 µL of blood was collected and repeat biotinylated RBC infusion was administered prior to the measurement of post-bleed blood volumes. The measured post-bleed blood volumes closely approximated the values expected based on the amount of blood removed (B, R = 0.98 and slope = 0.97 with dotted line indicating the line of identity).