Table 2. Blood Volume.

	WT	F508del	Pre-bleed	Post-bleed
N	10	6	4	4
Mean corpuscular volume (fL)	48.6±0.2	46.9±0.5	47.8±0.7	47.6±0.8
Hematocrit (%)	44±2	39±4	49±1	43±1*
Blood Volume (ml/kg)	58±3	64±2	64±3	60±4*

One hour after infusion of biotinylated RBCs, mean corpuscular volume and hematocrit were measured along with flow cytometric determination of the percentage of donor and recipient RBCs. These values were utilized in calculating the circulating blood volume. In 4 mice (3 WT and 1 F508del), an additional 200 µl of blood was collected and a second biotinylated RBC transfusion administered prior to the measurement of post-bleed values. *P<0.05 versus pre-bleed values by paired t-test.