Table 1. Affinity measurements of anti-gD variant binding to mFcRn by surface plasmon resonance.
Variant | kon (M−1s−1) | koff (s−1) | KD (nM) |
---|---|---|---|
IgG1 WT |
6160 | 0.0037 | 601 |
IgG1 FcRn+ |
19100 | 0.0021 | 108 |
IgG1 FcRn- |
1230 | 0.1146 | 93000 |
Variant | kon (M−1s−1) | koff (s−1) | KD (nM) |
---|---|---|---|
IgG1 WT |
6160 | 0.0037 | 601 |
IgG1 FcRn+ |
19100 | 0.0021 | 108 |
IgG1 FcRn- |
1230 | 0.1146 | 93000 |