Figure 2.Proliferation and cytokine release of human PBMC co-cultured with human endothelial cells in response to anti-CD28 mAbs. (A) Proliferation at 72 h, (B) IFNγ, (C) TNF,(D) IL-2, (E) IL-4, (F) IL-5, (G) IL-6 and (H) IL-12p70 release at 48 h after addition of 0.4, 2, 10 or 50 µg/ml of humanized pegylated Fab’ fragment FR104, divalent agonist anti-CD28.2 mAb and superagonist anti-CD28 ANC28.1 mAb or of an equivalent volume of excipient. Histogram bars are means ± SEM from data obtained with four different blood donors. LLOQ: Lower limit of quantification.