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. 2014 Feb 13;6(3):707–716. doi: 10.4161/mabs.28191

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Copyright © 2014 Landes Bioscience

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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graphic file with name mabs-6-707-g3.jpg — Figure 3. Mapping of the aldolase C region recognized by 9F mAb. (A) and (C) Scheme of the regions of the human aldolase C protein, cloned in the 3xFLAG CMV 7.1 vector. The corresponding clone names are reported on the right. The amino acid limits of each sub-region are indicated by numbers and colors. (B) western blot analysis of total protein lysates from Neuro2a cells alternatively transfected with the 3xFLAG tagged full-length (Full. L.), clone 1, clone 2 or clone 3 fusion products. Proteins were separated by 15% SDS-PAGE and probed with an anti-FLAG antibody (left panel) and with the anti-aldolase C 9F mAb (right panel). (D) western blot analysis of total protein lysates from Neuro2a cells alternatively transfected with the 3xFLAG tagged clone 1A and clone 1B fusion products or mock-transfected. Proteins were separated by 15% SDS-PAGE and probed with an anti-FLAG antibody (upper panel) and with the anti-aldolase C 9F mAb (lower panel). Alpha-tubulin served as loading control.