Figure 3. Anti-WEEV scFv-Fc antibody sandwich ELISA. Plates coated with a dilution series of WEEV suspensions captured by anti-WEEV mAb SFV12/2 (3 µg/mL). Three titration ELISAs were made in parallel. Staining was performed by incubation with 1 µg/mL biotinylated anti-WEEV antibodies, followed by streptavidin-HRP conjugate (1:4,000). As a negative control, we assessed antibody binding to Vero cell culture material.