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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Methods. 2014 Jan 15;67(2):169–176. doi: 10.1016/j.ymeth.2014.01.010

Fig. 1. Single molecule photobleaching assay for direct counting of biomolecules.

Fig. 1

(A) Left: A typical initial fluorescence image of individual biocomplexes containing multiple copies of fluorescently labeled molecules prior to photobleaching process. Right: Photobleaching trace of average fluorescent intensity vs. time for the fluorescent spot circled in the image on the left. (B) Schematic of the single molecule TIRF microscope. Adapted from [18] © 2007 with permission from Nature Publishing Group. (C–D) Schematic designs of the optics inside the (C) laser combiner and the (D) Dual-View Imager™. Adapted from [31] © 2010 with permission from Springer.