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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Methods. 2014 Jan 15;67(2):169–176. doi: 10.1016/j.ymeth.2014.01.010

Fig. 4. Dual-color photobleaching of differently labeled pRNAs within the same motor/pRNA complex.

Fig. 4

(A) Design of dual-labeled pRNA for motor binding. (B) Typical overlaid fluorescence image of the dual-color labeled motor/pRNA complexes. (Green: Cy3; Red: Cy5; Yellow: Cy3/Cy5 overlay). (C) Comparison of the experimental histogram of photobleaching steps with the theoretical histograms for Cy3-pRNA based on 70% labeling efficiency. (D) A dual-color photobleaching trace of motor/pRNA complex showing three Cy3-pRNA molecules and three Cy5-pRNA molecules on the same motor. Adapted from [18] © 2007 with permission from Nature Publishing Group.