Skip to main content
. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 7;192(10):4876–4886. doi: 10.4049/jimmunol.1302750

Figure 4. NFATc1, STAT3, STAT4, p300, and CTCF bind to the Pdcd1 locus.

Figure 4

Quantitative ChIP for NFATc1, STAT3, STAT4, p300 and CTCF binding to the regions indicated was performed. All ChIP data is presented as average percent input from three independent experiments with standard deviation. An IgG control antiserum was used as a specificity control for antibody binding. (A) ChIP for NFATc1, STAT3, STAT4, p300, or a control IgG from splenic CD8 T cells stimulated for 24h with CD3/CD28 beads, IL-6, and/or IL-12 as indicated. (B) Consensus CTCF binding sequence in logo format aligned with the DNA sequences from −3.7 and +17.1 is shown. (C) ChIP for CTCF or a control IgG from CD8 T cells stimulated with CD3/CD28 beads, or beads and IL-6 and EL4 cells stimulated with PMA/Io or PMA/Io (P/I) and IL-6.