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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Pharm Res. 2013 Dec 3;31(5):1315–1324. doi: 10.1007/s11095-013-1252-8

Table II.

LC/MS/MS method for C8-DALDA analysis

Condition Description
Mobile phase

  1. 0.1% formic acid in water

  2. 0.1% formic acid in methanol
Column

  1. Kinetix, C18, 2.6μm, 2mm x 50mm, 100 Å pore size

  2. Mixing column, 5 μm, Silica, 30X3 mm
Flow rate 0.5 ml/min
Injection volume 10μl
Detector API5000
Temperature Ambient
Pressure 312 bars, 5500 psi
Gradient run
Time (min) A (%) B (%)
0.00 80 20
3.00 10 90
4.50 10 90
4.51 80 20
6.00 80 20
Mass transitions Q1, Da Q3, Da
369.9 484.5
369.9 612.4