Table II.
Composition of the Fasted State Lipolysis Media as Used for Gastric and Intestinal In Vitro Lipolysis
| pH | Pepsin (mg/mL) | Bile saltsa (mM) | SPC (mM) | Maleate (mM) | NaCl (mM) | Lipase activity (U/mL)b | |
|---|---|---|---|---|---|---|---|
| Gastric | 2 | 0.15 | 0.080 | 0.020 | 2 | 34.2 | 10 |
| Intestinal | 6.5 | – | 3 | 0.6 | 10 | 70 | 179 |
SPC soybean phospholipids
aSodium taurodeoxycholate was used for the preparation of gastric medium; porcine bile extract for intestinal medium
bLipase activity in the gastric step was determined as tributyrin units and the activity in the duodenal step according to USP method (Christophersen et al. (20))