Fig. 5.

Subcellular localization of Plk1 at distinct subcellular structures. Immunostained HeLa cells show that Plk1 is abundantly localized at specific subcellular structures, such as centrosomes, kinetochores (marked with CREST), and midbodies. Like PBIP1-dependent Plk1 recruitment to kinetochores (Kang et al., 2006), whether the self-priming and binding mechanism operates to help promote Plk1 recruitment to centrosomes and midbodies remains to be further investigated. Merge, Plk1 (green) and CREST (red) signals overlaid with DAPI-stained chromosomes (blue); Asterisks, centrosomes; yellow-arrowed brac-ket, kinetochores; white-arrowed bracket, mizone.