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. 2014 Apr 8;37(4):330–336. doi: 10.14348/molcells.2014.0003

Fig. 2.

Fig. 2.

Site and sequences of the antisense morpholino oligomers used for blocking zTB4 splicing and the phenotype of zTB4 morphants. (A) The upper diagram shows zTB4 gene exons 2–4. zTB4 MO was designed to block splicing of exon 2 (ATG TCTGAC) and intron 2 (CTGCA AAC) of zTB4 pre-mRNA (resultant gene diagrams are indicated below MO sequences). (B) After MO (5 ng) injection into one cell embryo, the expression levels of zTB4 and β-actin mRNA were determined by RT-PCR in total RNA. (C) Morphologies of control embryos (a, c) and zTB4 MO-injected embryos (b, d) at 48 hpf were observed under a stereomicroscope. a, b; lateral view. Drawings inside pictures indicate the ventricle (V) and atrium (A) and the arrow indicates the heart tube. c, d; frontal view. Dotted lines show the heart tube. Tg(cmlc2:EGFP) embryos showing heart tube morphology in the dark images located inside the bright field pictures in (c, d).