Fig. 3.

Expression of endocardial and EMT marker genes in AVCs were disturbed in zTB4 morpholino-injected zebrafish embryos. (A) Whole mount in situ hybridization of embryos at 48 hpf was performed using riboprobes for cmlc2, bmp4, HAS2, and notch1b. Staining for cmlc2 (a, e, i), bmp4 (b, f, j), HAS2 (c, g, k), and notch1b (d, h, l) was observed in controls (a–d), zTB4 morholino (MO) (e–h), and MO plus zTB4 mRNA (i–l) injected embryos. (a′–l′) are schematic diagrams of the heart tube region (dashed line) and the expression patterns of each gene (red line).