Fig. 4.

sXBP1, IL-6 and TNF expression in SF following stimulation with LPS and SNAPIN. 4. (A–C) sXBP1 mRNA levels in RA SF measured by qRT-PCR after treatment with LPS (10 ng/ml) for 6 h, TLR4 inhibitor (1 μg/ml) for 30 min followed by LPS (10 ng/ml) for 6 h, SNAPIN (5 μg/ml) for 24 h, with or without prior TLR4 (1 μg/ml) and TLR2/4 (30 μg/ml) inhibition for 30 min, and Tg (300 nm/ml) for 6 h with or without TLR2/4 (30 μg/ml) treatment, with DMSO as vehicle control. Results are expressed relative to HPRT. Error bars represent the standard deviation of 3 independent experiments. (B–C) Supernatants were harvested from RA SF and IL-6 and TNF levels were determined by ELISA, the X-axis represents the different treatments described above. Error bars represent the standard deviation of 3 independent experiments.