Fig. 6.

sXBP1 expression in response to pro-inflammatory cytokines (A) sXBP1 mRNA levels in RA-SF determined by qRT-PCR following treatment with LPS (10 ng/ml) for 6 h, TNF, IL-1β, IL-6 (all used at concentration of 10 ng/ml) for 24 h (B) sXBP1 expression and subcellular localisation was determined by IF after SF were treated with Tg (1 μM for 3 h) as a positive control and TNF (10 ng/ml for 48 h). Blue (DAPI) = nucleus; green (FITC) = SYVN1; red (TRITC) = sXBP1. Images taken at ×640 magnification.