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. 2014 May;50(100):107–122. doi: 10.1016/j.jaut.2014.01.032

Fig. 2.

Fig. 2

High frequencies of CD25 expressing B cells (CD19+CD25+) were detected in blood circulation of the IL-33-treated mice. Groups of IL-10−/− and WT mice were injected i.p. with rhIL-33 (1 μg/mouse) twice a week, for 6 weeks starting at the age of 7 wk. The mice were bled weekly and the peripheral blood mononuclear cells (PBMC) analysed by flow cytometry using fluorescent conjugated antibodies specific for mouse CD19 and CD25. A. Selected representative FACS profiles of samples analysed at Week-2 after the 1st IL-33 injection. Kinetic changes in the frequency of CD19+CD25+ cells among total B cells (B) and of total lymphocyte population (C) were analysed. Data shown in B and C were pooled from 2 repeated experiments (n = 8–9). *p < 0.05; ***p < 0.001 (IL-33-treated WT versus IL-10−/− groups, Student's t test, 2 tailed).