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. 2014 May 1;13:54. doi: 10.1186/1475-925X-13-54

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Copyright © 2014 Girão-Silva et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Contraction assay of hASCs that underwent mechanical stimulation (12%, 1Hz). (A) Illustrative image from collagen gel discs with no cells and with endothelial cells (ECs), smooth muscle cells (SMCs), static hASCs or 96 hours stretched hASCs. ECs and SMCs from human saphenous vein were used as negative and positive controls of contraction, respectively. (B) Quantification of gel disc contraction was analyzed after 48 hours (hASCs, n = 7; SMCs n = 4; ECs, n = 2). Relative lattice area was obtained by measuring the final dimensions in comparison with its initial area. The bars represent means ± SD and * indicates p < 0.01 compared to 0 h. Sample sizes indicate data from independent experiments.