Table 2.
Oligonucleotides primers used for RT-PCR, product size and cycles used
| Target | Forward | Reverse | PCR product (bp) | Cycles |
|---|---|---|---|---|
| Calponin |
CAGATGGGCACCAACAAAG |
CATCTGCAGGCTGACATTGA |
123 |
29 |
| CD31 |
CCACTGCAGAGTACCAGCT |
CACCTTGGATGGCCTCTTTC |
80 |
35 |
| Cyclophilin |
ATGGTCAACCCCACCGTGT |
TCTGCTGTCTTTGGGACCTTGTC |
101 |
24 |
| GAPDH |
TGGTCTCCTCTGACTTCAACA |
AGCCAAATTCGTTGTCATACC |
118 |
24 |
| GATA4 |
CTGTCATCTCACTACGGGCA |
TAGCCTTGTGGGGAGAGCTT |
122 |
34 |
| KDR |
TCAGAAGAGCTGAAAACTT |
GAGCCTTCAGATGCCACAGA |
80 |
34 |
| MKL1 |
ACCGTGACCAATAAGAATGC |
CATCTGCTGGCTTGAGGAAC |
240 |
29 |
| MKL2 |
ATTTCCAACGCTCACAGTCA |
TTCACTGGCATTGTGGTGAT |
146 |
29 |
| Myocd |
TTCCTGTGGATTCTGCTGTG |
GGCTGTGAGGCTGAGTCATT |
262 |
34 |
| SM-MHC | CCATCCAGTTTCCTCTCCAC | GTCACTGAGTTGGCCCCTTCT | 86 | 34 |