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. 2014 Apr 30;5:165. doi: 10.3389/fpls.2014.00165

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Copyright © 2014 Kamiuchi, Yamamoto, Furutani, Tasaka and Aida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CMM development in CUC1g-m7 and CUC2g-m4. (A–H) In situ hybridization on transverse sections from developing gynoecia of CUC1g-m7 (A,B), CUC2g-m4 (C,D) and wild type Col (E–H) probed with CUC1 (A,B,E,F) and CUC2 (C,D,G,H). Staining reaction was carried out for 12 h in CUC1g-m7 (A,B) and CUC2g-m4 (C,D), and for 36 h in Col (E–H). Sections are prepared from gynoecia at stage 6 (A,C,E,G) and late stage 9 (B,D,F,H). (I–N) Transverse sections from developing gynoecia of CUC1g-m7 (I–K) and CUC2g-m4 (L–N) at early stage 9 (I,L), late stage 9 (J,M), and stage 11 (K,N). Black arrowheads indicate CMMs at normal positions and white arrowheads indicate those at abnormal positions. Arrow in (N) indicates a primordium of a filamentous structure. s, septum. Scale bars are 50 μm.