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. 2014 Apr 30;5:171. doi: 10.3389/fpls.2014.00171

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Copyright © 2014 Oh and Montgomery.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phytochrome-dependent regulation of SIG6 expression. (A) Expression levels (signal value) of SIG6 in No-0 wild-type (WT), 35S::pBVR3 (35S), and CAB3::pBVR2 (CAB3) under continuous far-red (FRc) are shown (±SD, n = 3). Signal value indicates signal intensity on the ATH1 array as calculated by Affymetrix Microarray Suite (MAS). (B) Validation of microarray data for SIG6 using quantitative RT-PCR (qRT-PCR) analysis. Relative SIG6 expression level compared to UBC21 is shown (±SD, n = 3). (C) qRT-PCR analysis of SIG6 expression in Col-0 WT, phyA (SALK_014575), phyB (SALK_022035), or phyA/phyB double mutant seedlings under FRc or continuous red (Rc). Relative SIG6 expression level compared to UBC21 is shown (±SD, n = 3). Statistics: Unpaired, two-tailed Student's t-test comparing mutants or transgenic lines to WT, ^**p < 0.01, ^***p < 0.005.