Figure 1.

Cytb5–KikGR aggregates were degraded by nutrient deprivation-induced autophagy in tobacco BY-2 cells. Transformed tobacco BY-2 cells expressing Cyt b5-KikGR at the exponential growth phase were incubated with normal control medium (A,E), sucrose-free medium (B,F), phosphate (Pi)-free medium (C,G), or nitrogen-free medium (D,H) and further cultured for 24 or 48 h. The epifluorescence image at 24 h (A–D) and migration pattern of KikGR fluorescent proteins on SDS–PAGE (E–H) at 0, 24, and 48 h of culture are shown. Each lane contains 5 μ g of protein from cell extracts. Black and white arrowheads indicate the migration positions of the intact and processed forms of Cyt b5-KikGR, respectively.