Figure 5.

Phosphite did not prevent the induction of autophagy under sucrose—or nitrogen-starvation. Color-converted cells expressing Cyt b5-KikGR were incubated in medium with or without one of the nutrients as described in the legend to Figure 1A with or without 2.6 mM Phi for the indicated times, and analyzed as in the legend to Figure 3A. Only a color-merged image is shown. Black and white arrowheads indicate the migration positions of the intact and processed forms of Cyt b5-KikGR, respectively.