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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Mar 23;21(4):383–388. doi: 10.1038/nsmb.2797

Figure 4. RGD-containing loop structures in wild type and modified FN10.

Figure 4

(a) Superimposed R/KGD-containing loops of hFN10, eptifibatide (pdb id 2vdn) and barbourin (pdb id 1q7j, model 2). Residues R/KGDWN common to hFN10 and barbourin are labeled in black and the three flanking residues are in the respective loop color. (b) Superimposed structures of RGD-containing loops of αVβ3-hFN10, αVβ3-wtFN10, barbourin and αVβ3-hFN10/B. The position the Cα and Cβ of Trp1496 in the αVβ3-hFN10/B complex is as that in barbourin or eptifibatide. (c) Main ionic interactions at the αVβ3-hFN10/B interface involving the RGD-containing loop (in dark cyan). (d) Binding (mean±SD, n=3 independent experiments) of fluoresceinated AP5 mAb to M21 cells in absence (control) and presence of wtFN10, hFN10 or hFN10/B.