Figure 7. ChIP-seq validation of SRF neighborhood. The vertical lines represent 30 kb sequence around transcription start sites (arrows point to the direction of transcription) of 9 predicted or known target genes of SRF. Read density profiles from a ChIP-seq experiment are shown for each gene. There is a pronounced region of high read density (“peak”) within the promoter showing autoregulation of SRF, regulation of actin genes (ACTA2, ACTB) and FHL2. SRF has not bound to the promoter of myocardin (MYOCD), one of the established targets of SRF. Predicted target PRUNE2 also shows a peak at their promoter region.