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. 2014 May 7;34(19):6495–6509. doi: 10.1523/JNEUROSCI.0073-14.2014

Figure 5.

Figure 5.

Analysis of sleep and wakefulness in orexin-tTA; TetO DTA mice. A, Schematic illustrating Experimental protocol 2. EEG and EMG electrodes were implanted at 10 weeks of age. Control recordings for orexin-tTA; TetO DTA mice occurred between 11 and 12 weeks of age. At 12 weeks of age, DOX was removed from the chow for the subsequent 13 weeks. In contrast, orexin-tTA; TetO DTA D(−) mice were raised from birth without DOX in the chow. In this strain (referred to as D(−) mice), EEG and EMG were recorded between 11 and 12 weeks as a comparison. Green bar represents DOX(+) condition; open bar represents DOX(−) condition. B, Number of orexin-ir cell bodies counted at the end of experiments. C, Example hypnograms from orexin-tTA; TetO DTA mice (control and 1, 2, 4, and 13 weeks in DOX(−) condition) and orexin/ataxin-3 (AT) mice. Clock time and light-dark period are indicated at the bottom of the hypnogram (light on 8:00–20:00 h; light off 20:00–8:00 h). D, Time spent in wakefulness, SWS, REM sleep, and cataplexy in the dark (top) and light (bottom) periods. In orexin-tTA; TetO DTA mice, 24 h recordings occurred at 1, 2, 3, 4, 7, 8, 9, 10, 11, and 13 weeks after DOX removal from the diet. *p < 0.05 versus control. Values are mean ± SEM (n = 5–10). D(−), orexin-tTA; TetO DTA mice raised from birth in DOX(−) condition. Ctr, Control; AT, orexin/ataxin-3 mice; Wake or W, wakefulness; SWS or S, slow-wave sleep; REM or R, REM sleep; C, cataplexy.