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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Methods Enzymol. 2013;529:227–240. doi: 10.1016/B978-0-12-418687-3.00018-5
Note Some of the stock solutions come with the pH indicator phenol red. This supplement does not affect the application and might be useful if the researcher wishes to visualize any pH changes that can occur in the solutions over time. In the case of non-CO2 incubators (e.g., when scaling-up the production of adherent cells in roller bottles), HEPES-buffered media can be used to keep the pH stable.
Note Catalog numbers are from the US website of Invitrogen and may differ on other local websites.