| PEI ‘Max’ | ||
| Dissolve 1 g PEI ‘Max’ in 900 ml distilled water. Adjust the pH to 7.0 with 1 N NaOH. Add distilled water to 1 l | ||
| Note: Stable at least 9 months at 4 °C | ||
| Make smaller volumes depending on how much is needed. PEI ‘Max’ cannot be frozen! | ||
| FreeStyle™ 293 ‘Completed’ | ||
| Component | Stock | Amount |
| FreeStyle™ 293 medium | 1 l | |
| FBS | 100% | 10 ml |
| l-Glutamine | 200 mM | 10 ml |
| DMEM:F12, 5% FBS | ||
| Add 50 ml FBS to 1 l of DMEM:F12 | ||
| Alpha MEM, 5% FBS | ||
| Add 50 ml FBS to 1 l of Alpha MEM | ||
| Hybridoma SFM, 1% FBS | ||
| Add 10 ml FBS to 1 l of Hybridoma SFM | ||
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