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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Brain Res. 2012 Sep 13;1483:31–38. doi: 10.1016/j.brainres.2012.09.013

Figure 4.

Figure 4

Exposure to METH significantly increases the number of striatal cells that co-localize cyclic GMP with activated caspase-3 and effect of NPY2R agonist. Control and experimental animals were given aCSF infusion into one striatum and 1.0 μl of 20μM NPY2R agonist into the contralateral striatum. Mice (n=6) received an injection of METH (30 mg/kg, ip) 30 minutes after the intrastriatal infusions and were sacrificed 8 hours after the injection. Activated caspase-3 and cyclic GMP were visualized in the same section of striatal tissue by immunofluorescence in the confocal microscope. Note that the number of striatal cells co-expressing these two markers increases nearly five-fold in the group exposed to METH and the NPY2R agonist significantly attenuates this effect of METH. Differences between groups were analyzed by t-test and Mann Whitney’s post-hoc test (**p<0.01).