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. Author manuscript; available in PMC: 2015 Apr 14.
Published in final edited form as: Cancer Cell. 2014 Apr 3;25(4):415–427. doi: 10.1016/j.ccr.2014.02.008

Figure 7. G2DHE drives EVI1 overexpression in HSPC.

Figure 7

(A-F) Representative flow cytometric profiles of Lin− cells (A), LSK cells (C) and LK cells (E) in 12-week-old Tg (−), 3q26 line B, 3q21q26 line B and 3q21q26ΔG2DHE line B mice. Cells in red boxes were analyzed in panels B, D and F. Absolute cell numbers of LSK and LK cells (B), LT-HSC, ST-HSC, MPP and CD34highFlt3− (CD34high) cells (D), and CMP, MEP and GMP cells (F) in the Lin− cell population in 12-week-old Tg (−) (white, n=4), 3q26 line B (blue, n=4), 3q21q26 line B (pink, n=4) and 3q21q26ΔG2DHE line B (green, n=4) mice are depicted. (G-I) Expression levels of human EVI1 mRNA (G), mouse endogenous Evi1 mRNA (H), and total EVI1 mRNA (I) in LT-HSC, ST-HSC, MPP, CD34high, CMP, MEP and GMP fractions in 12-week-old Tg (−) (white, n=4-8), 3q26 line B (blue, n=4-8), 3q21q26 line B (pink, n=4-8) and 3q21q26ΔG2DHE line B (green, n=4-8) mice. The expression level of each mRNA was normalized to rRNA abundance. Average values for 3q26 LT-HSC cells (G) and Tg (−) LT-HSC cells (H, I) were set to 1.

Bar graphs are represented as mean +/− SD. Arrows indicate undetectable or slight expression levels. N.D.; not determined. *, p<0.05; **, p<0.01 compared with Tg (−). #, p<0.05; ##, p<0.01.

See also Figure S4.