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. Author manuscript; available in PMC: 2015 Jan 8.
Published in final edited form as: Neuron. 2014 Jan 8;81(1):91–102. doi: 10.1016/j.neuron.2013.10.056

Table 2.

Synaptic bouton counts in “WT” and “S218L” flies.

Genotype Muscle A2 6/7 Muscle A3 6/7
“WT” 97.6 ± 3.2 (n=19) 79.7 ± 4.3 (n=19)
“S218L” 93.0 ± 3.2 (n=27)
p = 0.31		 74.5 ± 2.0 (n=26)
p = 0.28

Drosophila larval NMJ filet preparations were double-labeled with anti-synapsin and anti-Dlg primary antibodies as described in Experimental Procedures. Synapses for muscle group 6/7 in segments A2 and A3 were examined by immunofluorescence, and synaptic boutons per NMJ 6/7 were counted. For both muscle segments examined, presynaptic expression of “S218L” has no significant effect on synapse growth by Student’s t-test (compared to “WT”, p-values indicated). Numbers represent synapse counts (mean ± S.E.M.; n = number of synapses counted per genotype).