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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: In Vitro Toxicol. 1994;7(3):225–242.

FIG. 5.

FIG. 5

Slot-blot comparison of substrata and absence (no ECM) or presence of standard Matrigel (ECM) vs. growth-factor-reduced Matrigel (defined ECM) overlay on PB-inducibility of CYP3A1 gene expression. Hepatocytes were plated on collagen type 1, plastic or Primaria substrata and 48 h postisolation, treated with PB (0.1/1.00 mM) or BNF (22 μM) for 24 h. Total RNA was evaluated by slot-blot analysis, as stated in Materials and Methods section. The addition of ECM overlay (233 μg/ml) at 4 h postplating (4 h), and delaying the addition till 24 h postplating (24 h), also is shown.