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The amount of Sup35 is decreased in strains with deletion of SFP1 and is increased in [ISP+] strains comparatively to isogenic [isp−] strains. Detection of Sup35 by protein gel blot in strains that express the wild-type SUP35 allele and do not contain [ISP+] (A) and in [ISP+], [isp−] and sfp1Δ strains expressing the sup35-25 (B). Polyclonal antibodies against Sup35 were used.
