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. Author manuscript; available in PMC: 2014 Oct 3.
Published in final edited form as: Nature. 2014 Mar 16;508(7494):108–112. doi: 10.1038/nature13110

Figure 1. Nutrostat design and metabolic characterization of cancer cells under chronic glucose limitation.

Figure 1

a, Nutrostat Schematic. b, Fold change in cell number (top) and media glucose concentration (bottom) of Jurkat cells grown in Nutrostats at 10 mM (black) or 0.75 mM (blue) glucose. DT = doubling time. c, Indicated metabolite levels in Nutrostats at 10 mM (black) or 0.75 mM (blue) glucose. d, Differential intracellular metabolite abundances (p < 0.05) from cells in Nutrostats at 10 mM (bottom three rows) or 0.75 mM (top three rows) glucose. Color bar indicates scale (Log2 transformed). Error bars where shown are SEM (n=2 (glucose and lactate), 3 (NAD(H) ratio) and 8 for ATP levels). Replicates are biological, means reported. Asterisks indicate significance p < 0.05 by two-sided student’s t-test.