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. 2014 Apr 2;12(4):1939–1958. doi: 10.3390/md12041939

Figure 3.

Figure 3

Dicitrinone B induces significant apoptotic morphological changes. (A) After being exposed to zero, five, 10 and 20 μM of dicitrinone B for 24 h, A375 cells were stained by Hoechst 33258 or acridine orange/ethidium bromide (AO/EB). (B) Quantification of the proportion of apoptotic cells detected by Hoechst 33258 staining and AO/EB staining. Data are presented as the mean ± SD from three independent experiments. Significant differences between the treatment and control groups are indicated as * (p < 0.05) or ** (p < 0.01).