Figure 7.

Analysis of the apoptosis-associated caspase activities in A375 cells. (A) A375 cells were treated with dicitrinone B (zero, five, 10 and 20 μM) for 24 h. The activities of caspase-9, -8 and -3 were determined by colorimetric assays kits, respectively. (B) A375 cells were incubated with 20 μM of dicitrinone B in the presence or absence of 20 μM of the caspase inhibitor, Z-VAD-FMK and then analyzed with Annexin-V/PI staining by flow cytometry. (C) Densitometry of cell counts. Data are expressed as the mean ± SD (n = 3). Significant differences between the treatment and control groups are indicated as * (p < 0.05) or ** (p < 0.01).