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. 2014 Apr 3;12(4):2054–2065. doi: 10.3390/md12042054

Figure 4.

Figure 4

Placotylene A (1) abolishes RANKL-stimulated NFATc1 transcriptional and translational expression. (A) BMMs were pretreated with DMSO or 1 (10 μM) for 30 min and then stimulated with RANKL (10 ng/mL) for the indicated number of days. The expressed mRNA levels were analyzed by comparing real-time PCR with the DMSO control. ** P < 0.05, *** P < 0.005. (B) BMMs were pretreated with DMSO or 1 (10 μM) for 1 h and then stimulated with RANKL (10 ng/mL) for the indicated time. The cell lysates were analyzed by western blotting with anti-NFATc1, and anti-actin antibodies as indicated.