Figure 5.

Placotylene A (1)-mediated inhibition of osteoclast differentiation was restored by NFATc1 over-expression. (A) BMMs were transduced with the indicated retroviruses harboring vehicle with GFP tag or Ca-NFATc1 expression construct with GFP tag using a retroviral method for 24 h. Infected BMMs were cultured with M-CSF (30 ng/mL) and RANKL (10 ng) for 4 days in the presence or absence of placotylene A (10 μM). The expressed GFP of the infected cells was visualized by fluorescence microscope. (B) TRAP-positive multinucleated osteoclasts were visualized by TRAP staining. (C) TRAP positive cells were counted as osteoclasts (nuclei ≥ 3). ** P < 0.01. (D) TRAP activity was measured at 405 nm. ** P < 0.01.