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. 2014 Apr 10;12(4):2205–2227. doi: 10.3390/md12042205

Table 4.

Polymer-H assay-based test concept for identification and differentiation of sulfated poly- and oligosaccharides. By testing four concentrations, the Polymer-H assays allows distinction between structurally different sulfated poly- and oligosaccharides by means of the three parameters FI increase at 7.5 µg/mL, linear range and slope of the line. By inclusion the data of a reference sulfated polysaccharides (SP), the considered SP can be identified. For identification of closely related SP, additional characteristics such as the sulfate content and the elastase inhibitory activity (IC50) have to be included.

Substances Polymer-H assay Sulfate content Elastase inhibition (IC50) Distinction from D.s.-SP by additional parameters
Mean FI increase at 7.5 µg/mL (%) Linearity 1 (1.0 to 7.5 µg/mL) slope of the line 2 (rel. to D.s.-SPref) Distinction from D.s.-SP
1 2 3 1 2 3 (% m/m) (µg/mL) PolyH/other
D.s.-SP 80–105 yes 1.00 ± 0.11 - 20.4 ± 1.6 0.20 ± 0.02 -
D.s.-SPref 92 ± 7 yes 1.00 - 20.5 0.19 -
Structurally different oligo-and polysaccharides
Fondaparinux 110 no 2.47 + + + 41.5 >10 +
Heparin 107 no 2.06 + + + 27.7 0.31 +
Phycarin 0 no 0.0 + + + 0.0 >25 +
Agarose 0 no 0.0 + + + ≤0.2 >6.25 +
Fucoidan 63 no 0.68 + + + 20.8 0.69 +
Alginate sodium 91 no 1.47 + + 0.0 >2.5 +
λ-carrageenan 106 (yes) 1.08 (+) 3 44.0 0.22 + 3/SC
κ-carrageenan 85 (yes) 0.85 (+) 3 20.7 >6.25 + 3/IC50
Red algae polysaccharides structurally related to D.s.-SP
D.s.-SP 7.1 93 yes 0.92 20.2 0.19 -
P.r.-SP 113 yes 1.18 + (+) 3 21.0 0.19 +
P.p.-SP 99 yes 1.01 16.7 0.45 −/SC, IC50
C.t.-SP 72 yes 0.73 + + 16.0 0.18 +

1 linearity: r > 0.997; 2 slope of the line in the range of 1.0–7.5 µg/mL, except for fondaparinux (FPX) and heparin: 1.0–3.75 µg/mL, fucoidan and alginate sodium: 1.0–5.0 µg/mL; 3 by the FI values at 2.5 and 5.0 µg/mL, distinction from D.s.-SP is possible.