Table 4.
Substances | Polymer-H assay | Sulfate content | Elastase inhibition (IC50) | Distinction from D.s.-SP by additional parameters | |||||
---|---|---|---|---|---|---|---|---|---|
Mean FI increase at 7.5 µg/mL (%) | Linearity 1 (1.0 to 7.5 µg/mL) | slope of the line 2 (rel. to D.s.-SPref) | Distinction from D.s.-SP | ||||||
1 | 2 | 3 | 1 | 2 | 3 | (% m/m) | (µg/mL) | PolyH/other | |
D.s.-SP | 80–105 | yes | 1.00 ± 0.11 | - | 20.4 ± 1.6 | 0.20 ± 0.02 | - | ||
D.s.-SPref | 92 ± 7 | yes | 1.00 | - | 20.5 | 0.19 | - | ||
Structurally different oligo-and polysaccharides | |||||||||
Fondaparinux | 110 | no | 2.47 | + | + | + | 41.5 | >10 | + |
Heparin | 107 | no | 2.06 | + | + | + | 27.7 | 0.31 | + |
Phycarin | 0 | no | 0.0 | + | + | + | 0.0 | >25 | + |
Agarose | 0 | no | 0.0 | + | + | + | ≤0.2 | >6.25 | + |
Fucoidan | 63 | no | 0.68 | + | + | + | 20.8 | 0.69 | + |
Alginate sodium | 91 | no | 1.47 | − | + | + | 0.0 | >2.5 | + |
λ-carrageenan | 106 | (yes) | 1.08 | (+) | − | − 3 | 44.0 | 0.22 | + 3/SC |
κ-carrageenan | 85 | (yes) | 0.85 | − | − | (+) 3 | 20.7 | >6.25 | + 3/IC50 |
Red algae polysaccharides structurally related to D.s.-SP | |||||||||
D.s.-SP 7.1 | 93 | yes | 0.92 | − | − | − | 20.2 | 0.19 | - |
P.r.-SP | 113 | yes | 1.18 | + | − | (+) 3 | 21.0 | 0.19 | + |
P.p.-SP | 99 | yes | 1.01 | − | − | − | 16.7 | 0.45 | −/SC, IC50 |
C.t.-SP | 72 | yes | 0.73 | + | − | + | 16.0 | 0.18 | + |
1 linearity: r > 0.997; 2 slope of the line in the range of 1.0–7.5 µg/mL, except for fondaparinux (FPX) and heparin: 1.0–3.75 µg/mL, fucoidan and alginate sodium: 1.0–5.0 µg/mL; 3 by the FI values at 2.5 and 5.0 µg/mL, distinction from D.s.-SP is possible.