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. 2014 May;197(1):1–18. doi: 10.1534/genetics.113.154344

Table 1. Resources for Drosophila in vivo functional genomics studies (fly stocks).

Reagent type Source Recommended online entry point(s) References
Classical mutant alleles Community at large http://flybase.org/ Cook et al. (2010a); McQuilton et al. (2012); St Pierre et al. (2014); Yamamoto (2010)
http://flystocks.bio.indiana.edu/
http://www.dgrc.kit.ac.jp/
Transposon insertions (of various types and uses) Community at large http://flybase.org/ Cook et al. (2010a)
http://flystocks.bio.indiana.edu/ McQuilton et al. (2012); St Pierre et al. (2014); Yamamoto (2010)
http://www.dgrc.kit.ac.jp/
Transposon insertions (gene disruption collection) Drosophila Gene Disruption Project http://flypush.imgen.bcm.tmc.edu/pscreen/ Bellen et al. (2004, 2011); Venken et al. (2011)
RNAi Vienna Drosophila RNAi Center http://www.flyrnai.org/up-torr/ Dietzl et al. 2007; Hu et al. 2013a)
http://stockcenter.vdrc.at/control/main
RNAi Transgenic RNAi Project (TRiP) http://www.flyrnai.org/up-torr/ Hu et al. (2013a); Ni et al. (2011)
http://www.flyrnai.org/trip
http://www.flyrnai.org/rsvp Cook et al. (2010a)
http://flystocks.bio.indiana.edu/
RNAi NIG, Japan http://www.flyrnai.org/up-torr/ Hu et al. (2013a); Yamamoto (2010)
www.shigen.nig.ac.jp/fly/nigfly
RNAi Community at large http://flybase.org/ Cook et al. (2010a); McQuilton et al. (2012); St Pierre et al. (2014)
http://flystocks.bio.indiana.edu/
RNAi rescue with D. pseudoobscura genome fragments FlyFos project https://transgeneome.mpi-cbg.de Langer et al. (2010)
Overexpression (UAS) FlyORF resource http://flyorf.ch/ Bischof et al. (2013)
Overexpression (UAS) DPiM fly stocks https://interfly.med.harvard.edu/transgenic_info.php Guruharsha et al. (2011)
Gal4 expression Community at large http://flystocks.bio.indiana.edu/Browse/gal4/gal4_main.htm Cook et al. (2010a); Yamamoto (2010)
http://kyotofly.kit.jp/cgi-bin/stocks/data_search.cgi#GAL4
Gal4 expression Janelia Farms FlyLight project http://flystocks.bio.indiana.edu/Browse/gal4/gal4_Janelia.php Cook et al. (2010a)
Gal4 expression Vienna Tiles http://stockcenter.vdrc.at/control/vtlibrary Jenett et al. (2012)
Fluorescence protein trap Cooley and Chia laboratories http://flytrap.med.yale.edu/ Kelso et al. (2004); Morin et al. (2001); Yamamoto (2010)
http://kyotofly.kit.jp/cgi-bin/stocks/data_search.cgi#FlyTrap
Fluorescence protein trap Cambridge Protein Trap Insertion http://kyotofly.kit.jp/stocks/documents/CPTI.html Yamamoto (2010)
Fluorescence protein trap Carnegie Protein Trap Library http://flytrap.med.yale.edu/ Buszczak et al. (2007)
Fluorescence protein trap for dominant phenotypes Edwards laboratory collection Singari et al. (2014)
MiMIC insertions (for fluorescence trap, etc.) Drosophila Gene Disruption Project http://flypush.imgen.bcm.tmc.edu/pscreen/mimic.html Venken et al. (2011)
Human disease relevant (curated) Community at large http://flystocks.bio.indiana.edu/Browse/HD/HDintro.htm Cook et al. (2010a)
Human disease-relevant fly stocks (RNAi) TRiP http://www.flyrnai.org/hudis-trip Hu et al. (2011); Ni et al. (2011)
http://www.flyrnai.org/diopt-dist (after a search, click the button “find RNAi reagents” to carry the list to UP-TORR)
CRISPR/Cas9 system Kondo and Ueda http://www.shigen.nig.ac.jp/fly/nigfly/cas9/ Kondo and Ueda (2013)
Population genomics and analysis of quantitative traits Drosophila Genetic Reference Panel http://mackay.gnets.ncsu.edu/MackaySite/DGRP.html Mackay et al. (2012)
http://dgrp.gnets.ncsu.edu/
Non-melanogaster species Community at large php
http://kyotofly.kit.jp/cgi-bin/ehime/index.cgi