Table 2. Resources for Drosophila cell-based and molecular approaches (cell lines, plasmid clones, design tools, etc.).

Reagent type	Facility or lab group	Recommended online entry point(s)	References (PMID)
Cultured cell lines	Community at large	https://dgrc.cgb.indiana.edu	Cherbas and Gong (2014)
Cell-based RNAi libraries and reagents	DRSC	http://www.flyrnai.org/up-torr/	Flockhart et al. (2012); Hu et al. (2013a)
		http://www.flyrnai.org/
Cell-based RNAi reagent design	DRSC	www.flyrnai.org/snapdragon	Flockhart et al. (2012)
Cell-based RNAi reagent design	DKFZ/Boutros lab	www.dkfz.de/signaling/e-rnai3	Arziman et al. (2005); Horn et al. (2010)
		http://b110-wiki.dkfz.de/signaling/wiki/display/nextrnai/NEXT-RNAi
cDNAs and ORFs	BDGP	https://dgrc.cgb.indiana.edu	Stapleton et al. (2002)
pUAS-ORFs	BDGP and DRSC	http://plasmid.med.harvard.edu	Zuo et al. (2007)
Empty vectors, additional cDNAs or ORFs, etc.	Community at large	https://dgrc.cgb.indiana.edu	Herscovitch et al. (2012); Stapleton et al. (2002)
		http://www.addgene.org/
		http://plasmid.med.harvard.edu	Seiler et al. (2014); Zuo et al. (2007)
		https://dnasu.org/DNASU/Home.do
Plasmids for making CRISPR genome engineering constructs	AddGene	http://www.addgene.org/CRISPR/	Herscovitch et al. (2012)
Plasmids for making TALEN genome engineering constructs	AddGene	http://www.addgene.org/talengineering/	Herscovitch et al. (2012)
Primer designs for qPCR	DRSC	http://www.flyrnai.org/flyprimerbank	Hu et al. (2013b)
shRNA/siRNA design	DSIR	http://biodev.extra.cea.fr/DSIR/	Vert et al. (2006)

DSIR, Designer of Small Interfering RNA