Table 1. TALEN mutation frequencies in larvae.
| Gene/TALEN pairs(s) | [TALEN mRNA]a (ng/µl) | Total no. of screened larvae | No. of larval samples screened (n = no. of pooled/sample) | Mutation frequency (% mutation-positive samples) | % mutant genome copies/sample (range)b | % mutant genome copies/sample (average ± SD) |
|---|---|---|---|---|---|---|
| erEx3_L2/_R2 | 100 | 144 | 36 (n = 4) | 0.0 | 0 | 0 |
| erEx3_L2/_R2 | 180 | 20 | 5 (n = 4) | 20.0 | Not measured | NA |
| erEx3_L2/_R2 | 200 | 48 | 12 (n = 4) | 50.0 | Not measured | NA |
| erEx3_L2/_R2 | 267 | 68 | 17 (n = 4) | 70.6 | 2.94 - 6.08 | 4.98 ± 1.44 |
| erEx3_L2/R2 | 300 | 92 | 23 (n = 4) | 39.1 | 5.7–13.1 | 9.02 ± 3.2 |
| erEx2_L/_R + erEx3_L2/_R2 | 300 | 44 | 11 (n = 4) | 18.2 (deletion) | 22.3–27.3 (large deletion) | 24.8 ± 3.5 (large deletion) |
| l-cryEx2_L/_R + l-cryEx3_L/_R | 300 | 186 | 54 (n = 4 × 44; n = 1 × 10) | 18.5 (uncut bands); 16.7 (large deletions) | 2.21–8.84 (uncut bands); 12.7–56.2 (large deletions) | 5.33 ± 2.50 (uncut bands); 35.7 ± 18.9 (large deletions) |
| vtnEx2_L/R | 40 | 18 | 18 (n = 1) | 5.6 | Not measured | NA |
| vtnEx2_L/R | 100 | 32 | 8 (n = 4) | 75.0 | Not measured | NA |
| vtnEx2_L/R | 200 | 44 | 11 (n = 4) | 27.3 | 0.9–21.8 | 7.45 ± 9.00 |
a
Final concentration of mRNA in a 10-μl injection solution per TALEN.
b
Calculated from intensity of uncut or deletion band for samples of pooled larvae (n = 4–5). See also Figure S5, A and B.