Table 2. Mutation frequencies in adult worms and germline carriers in adult worms.
Gene/TALEN pair(s) | No. of worms raised | No. of mature adults screened | No. of mutation-positive adults | Mutation frequency (% mutation-positive adults) | % mutant genome copiesa | No. of germline carriers (carrier ID) |
---|---|---|---|---|---|---|
erEx2_L/R + erEx3_L2/R2 | 60 | 60 | 6 | 5% (3/60 indels); 3% (2/60: large deletion); 1% (1/60: inversion) | 10–23% (indels) 6–12% (large deletions) <1% (inversion) | 2 (er+32; er+59: large deletions) |
erEx3_L2/R2 | 6 | 6 | 0 | 0% | NA | NA |
l-cryEx2_L/R + l-cryEx3_L/R | >80 | 62 | 27 | 43% (27/60: indels) 9% (6/60: large deletions and indels) | 5–∼100% (indels) Not measured | 1 (l-cry+36: indels and large deletion) |
vtnEx2_L/R | 13 | 13 | 3 | 20% (indels) | Not measured | 0 |
Calculated from intensity of uncut or deletion band. Indicates individual mutation rate. See also Figure S5C.