Table 4.

Linearity and dynamic range of several metabolite standards.

Metabolite	Silylation (TMS)			Alkylation (MCF)
	Dynamic range pmol/sample	Linearity pmol/sample	r2*	Dynamic range pmol/sample	Linearity pmol/sample	r2*
ALA	≤ 10–400	10–80	0.97	25–200	25–200	1.00
ASP	40– ≥ 630	40–80	0.81	≤ 10 ≥ 400	25–100	0.98
AKG	40 ≥ 630	NL	-	25 ≥ 2000	25–200	0.98
CIT	≤ 10–400	10–80	0.98	≤ 10– ≥ 400	25–200	0.97
COU	25–500	20–80	0.98	≤ 10– ≥ 400	25–150	0.98
CYS	40– ≥ 400	40–80	0.77	25– ≥ 1000	25–200	0.98
FER	40–400	20–80	0.92	25– ≥ 400	25–200	0.99
FUM	40– ≥ 630	40–80	0.90	≤10– ≥ 600	25–200	0.99
GLN	40– ≥ 630	20–80	0.95	25– ≥ 1000	25–200	0.99
GLU	40– ≥ 630	40–80	0.73	25– ≥ 1000	25–100	0.98
ICI	≤ 10–400	10–80	0.99	25– ≥ 1000	25–150	0.98
LAC	≤ 10– ≥ 630	10–80	0.99	≤ 10– ≥ 400	25–100	0.99
LYS	60–500	NL	-	25–800	25–200	0.99
MET	60– ≥ 400	NL	-	25– ≥ 1000	25–200	0.99
MYR	40–400	40–80	0.91	25– ≥ 800	100–200	0.96
NAD+	nd	NL	-	25–1000	25–200	0.99
NADP+	nd	NL	-	25–1000	50–150	0.94
OAA	60–630	NL	-	50–2000	25–200	0.96
OIVAL	≤ 10– ≥ 630	10–80	0.99	≤ 10–1000	25–100	0.99
PAL	40–400	20–80	0.94	≤ 10– ≥ 1000	25–75	0.97
PEP	nd	NL	-	25– ≥ 1000	25–200	0.99
PHE	40– ≥ 630	40–80	0.71	≤ 10– ≥ 400	25–100	0.99
SUC	40- ≥ 630	40–80	0.91	≤ 10– ≥ 400	25–200	0.99
TRP	nd	NL	-	25–400	25–200	0.99
VAL	≤ 10– ≥ 630	10–80	0.98	≤ 10–200	25–200	0.99
2HB	≤ 10– ≥ 630	10–80	1.00	≤ 10– ≥ 1000	25–200	0.99

^*Regression coefficient from calibration line within the linear range shown in the table. NL, non-linear; nd, not determined. See Table 1 for metabolite abbreviations.