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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Cell Physiol. 2013 Oct;228(10):1996–2005. doi: 10.1002/jcp.24362

Figure 1. Tyrosine phosphorylation of CD95 is essential for increased cell killing.

Figure 1

Figure 1

(A) HuH7 cells were transfected with plasmids to express GFP, GFP-CD95 or GFP-CD95 YY-FF. Twenty four h after transfection cells were treated with vehicle (DMSO) or the indicated concentrations of sorafenib (Sor, µM) and/or vorinostat (Vor, 500 nM). Cells were isolated 24h after drug exposure and cell viability determined by trypan blue exclusion (n = 3 +/− SEM). *p < 0.05 greater than vehicle control; **p < 0.05 greater than corresponding value in vehicle control treated cells; #p < 0.05 less than corresponding values in CD95-GFP expressing cells.(B) HuH7 cells were transfected with plasmids to express GFP, GFP-CD95 or GFP-CD95 YY-FF. Twenty four h after transfection cells were treated with vehicle (DMSO) or the indicated concentrations of sorafenib (Sor, µM) and/or Na valproate (Val, 1 mM). Cells were isolated 24h after drug exposure and cell viability determined by trypan blue exclusion (n = 3 +/− SEM). *p < 0.05 greater than vehicle control; **p < 0.05 greater than corresponding value in vehicle control treated cells; #p < 0.05 less than corresponding values in CD95-GFP expressing cells.