Table I. Percentage of the cell surface (as surface area) covered by new HG.

JIM5-labeled cells were incubated for 48 h in the conditions listed. After this time, nonlabeled areas (the zones of newly deposited HG) in the wall were measured, surface areas were determined (Domozych et al., 2011), and percentage values were calculated per whole cell surface area. At least three independent sets of 100 cells per treatment were calculated and averaged.

Treatment	Percentage Surface Area of New Wall per Cell
Control (250 µm CaCl2)	48.2 ± 2.5
10× Ca2+ (2.5 mm CaCl2)	51.3 ± 2.7
One-tenth Ca2+ (25 µm CaCl2)	52.9 ± 3.5
3 units mL−1 PL	63.8 ± 3.0
250 µg mL−1 PME	32.0 ± 3.2
1 unit of arabinase	55.1 ± 3.2
P16 100 µg mL−1	42.5 ± 2.6
P70 100 µg mL−1	60.4 ± 3.0