Table I. BsaI/ccdB-based vectors for direct cloning of amiRNAs and syn-tasiRNAs.

CaMV, Cauliflower mosaic virus; nos, nopaline synthase; rbcS, Rubisco small subunit.

Vector	Small RNA Class	Bacterial Antibiotic Resistance	Plant Antibiotic Resistance	Gateway Use	Backbone	Promoter	Terminator	Plant Species Tested
pENTR-AtMIR390a-B/c	amiRNA	Kanamycin	–	Donor	pENTR	–	–	–
pFK210B-AtMIR390a-B/c	amiRNA	Spectinomycin	BASTA	–	pGreen III	CaMV 35S	rbcS	Arabidopsis
pMDC123SB-AtMIR390a-B/c	amiRNA	Kanamycin	BASTA	–	pMDC123	CaMV 2× 35S	–	Arabidopsis
								N. benthamiana
pMDC32B-AtMIR390a-B/c	amiRNA	Kanamycin	Hygromycin	–	pMDC32	CaMV 2× 35S	nos	Arabidopsis
		Hygromycin						N. benthamiana
pENTR-AtTAS1c-B/c	syn-tasiRNA	Kanamycin	–	Donor	pENTR	–	–	–
pMDC123SB-AtTAS1c-B/c	syn-tasiRNA	Kanamycin	BASTA	–	pMDC123	CaMV 2× 35S	nos	N. benthamiana
		Hygromycin
pMDC32B-AtTAS1c-B/c	syn-tasiRNA	Kanamycin	Hygromycin	–	pMDC32	CaMV 2× 35S	nos	Arabidopsis
		Hygromycin						N. benthamiana