Figure 2.

Phenotypic and molecular analysis of miROE lines. A, Phenotypes of spikelets and seeds in miROE and ZH10 plants. White arrowheads indicate florets with open husks or long sterile lemmas. Numbers indicate the ratio of open-husk or long sterile lemma florets. Bars = 3 mm. B, Expression levels of mature OsmiR396d were detected by qRT-PCR (n = 3; means ± sds) and small RNA blots (arrowhead indicates miR396). C, Transcription levels of OsGRF genes in 2-week-old seedlings of ZH10 and miROE8 were analyzed by qRT-PCR. *, Significant difference at P ≤ 0.05 compared with ZH10 by Student’s t test. (n = 3; means ± sds).